Method of preparing a eukaryotic viral vector

Details Method of preparing a eukaryotic viral vector Method of preparing a eukaryotic viral vector

2005-06-21

2005-06-21

Housel, James (Department: 1648)

Chemistry: molecular biology and microbiology

Vector, per se

C435S091330, C435S091320, C435S091410, C435S091420, C435S091400, C435S325000, C435S199000, C424S199100, C424S233100, C424S205100

Reexamination Certificate

active

06908762

ABSTRACT:
The inventive method of producing a eukaryotic viral vector comprises contacting a eukaryotic cell, which comprises a unique enzyme that nicks or cleaves a DNA molecule, with a recombinant phage vector, or contacting a eukaryotic cell, which does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves a DNA molecule, and (ii) a recombinant phage vector. The recombinant phage vector comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising a coding sequence, (b) a phage packaging site that is not contained within the eukaryotic viral vector genome, and (c) a promoter that is operably linked to the coding sequence.Alternatively, the DNA molecule is not present within the recombinant phage vector. The eukaryotic cell is contacted with the first DNA molecule and a recombinant phage vector. The first DNA molecule comprises a replication deficient eukaryotic viral vector genome comprising at least one adenoviral inverted terminal repeat and a packaging signal. The recombinant phage vector comprises a second DNA molecule and a phage packaging site, wherein the second DNA molecule complements in trans the replication deficient eukaryotic viral vector genome.The DNA molecule(s) enter the eukaryotic cell, and the unique enzyme nicks or cleaves the DNA molecule in the eukaryotic cell in at least one region not contained within the eukaryotic viral vector genome, thereby inducing the production of and ultimately producing a eukaryotic viral vector.

REFERENCES:
patent: 4578079 (1986-03-01), Ruoslahti et al.
patent: 5559099 (1996-09-01), Wickham et al.
patent: 5712136 (1998-01-01), Wickham et al.
patent: 5736388 (1998-04-01), Chada et al.
patent: 5846782 (1998-12-01), Wickham et al.
patent: 5851806 (1998-12-01), Kovesdi et al.
patent: 5962311 (1999-10-01), Wickham et al.
patent: 5994106 (1999-11-01), Kovesdi et al.
patent: 6329200 (2001-12-01), McVey et al.
patent: WO 95/34671 (1995-12-01), None
patent: WO 96/25506 (1996-08-01), None
patent: WO 97/00326 (1997-01-01), None
patent: WO 97/20051 (1997-06-01), None
patent: WO 97/21826 (1997-06-01), None
patent: WO 98/56937 (1998-12-01), None
patent: WO 99/02647 (1999-01-01), None
patent: WO 99/11764 (1999-03-01), None
patent: WO 99/15683 (1999-04-01), None
patent: WO 99/15684 (1999-04-01), None
patent: WO 00/14269 (2000-03-01), None
Life Science Dictiory edited by Biotech Resources and Indiane University, 1995-1998.
Brough et al.,J. Virol., 70, 6497-6501 (Sep. 1996).
Castro-Peralta et al.,Gene Ther., 7, 583-586 (2000).
Horwitz, “Adenoviruses,”in Fields Virology, 3rdEdition (vol. 2), Chapter 68, 2149-2171 (Fields et al., eds.) (Lippincott-Raven Publishers, New York, NY, 1996).
Kojima et al.,Biochem. Biophys. Res. Comm., 246, 868-872 (May 1998).
Miyake et al.,PNAS USA, 93, 1320-1324 (Feb. 1996).

Affiliated with

Also associated with

Rating

Method of preparing a eukaryotic viral vector does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of preparing a eukaryotic viral vector, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of preparing a eukaryotic viral vector will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3493712