Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1995-04-27
1996-09-17
Adams, Donald E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
4352401, 4352403, 435244, 4352402, 43524025, 43524026, 43524027, 530350, 530413, 530417, C07K 114, C07K 116, C07K 122, C12P 2100
Patent
active
055567703
ABSTRACT:
The present invention provides a composition of matter useful for enhancing the viability of hybridomas in culture which comprises a partially purified, cell-free extract derived from a medium conditioned by mitogen-stimulated macrophages, the extract being substantially free of the macrophage stimulating mitogen which had been added to the medium, and having within it a component characterized by the ability to enhance the viability of hybridomas in culture, and by an apparent molecular weight in the range from about 35,000 to about 45,000. Also provided are methods of preparing the composition of matter, and methods for enhancing the viability of hybridomas in culture.
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Hill Ruth B.
Sugasawara Renee J.
Adams Donald E.
IGEN, Inc.
Igoe Patrick J.
Ryan John W.
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