Method of preparing a composition containing a high amount of ga

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 172, 536 179, 536124, 536127, C07H 1500, C07H 108

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058441042

ABSTRACT:
A method of preparing a composition containing a high amount of Ganglioside which comprises the steps of: (1) adding ethanol to a material containing Ganglioside so as to have a concentration of ethanol of 30 to 70 volume % and recovering the precipitated fraction containing Ganglioside; (2) adding ethanol to the recovered fraction containing Ganglioside so as to have a concentration of ethanol of 70 to 90 volume % and removing the precipitated proteins; and, (3) treating the fraction containing Ganglioside from which the proteins have been removed, with a membrane.

REFERENCES:
patent: 4710490 (1987-12-01), Catsimpoolas et al.
Jennemann et al. Lipids 1994, 29(5), 365-368.
K. Takamizawa, M. Iwamori, M. Mutai and Y. Nagai, Gangliosides of Bovine Milk, J. Biological Chemistry, Apr. 25, 1986, vol. 261, pp. 5625-5630.
H. Kawakami, Y, Ishiyama and T. Idota, Stability of Milk Gangliosides And Formation Of G.sub.D3 Lactone Under Natural Acidic Conditions, Biosci. Biotech. Biochem., vol. 58, 1994, pp. 1314-1315.

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