Method of preparing a chromatography matrix

Details Method of preparing a chromatography matrix Method of preparing a chromatography matrix

2004-10-13

2008-07-08

Therkorn, Ernest G (Department: 1797)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S656000, C210S198200, C210S502100

Reexamination Certificate

active

07396467

ABSTRACT:
The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.

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Gel Filtration—Principles and Methods; Handbook from Pharmacia LKB Biotechnology, 5th Edition, 1991, pp. 41-42.

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