Method of preparing a biological material for use in ophthalmolo

Details Method of preparing a biological material for use in ophthalmolo Method of preparing a biological material for use in ophthalmolo

1998-11-17

1999-08-10

Lilling, Herbert J.

Compositions

Compositions containing a single chemical reactant or plural...

Organic reactant

25218212, 264 11, 264 138, 264 26, 264 28, 264 41, 435 681, 435201, 351160H, 514 54, 523105, 523106, 523108, 530356, 623 4, 623 6, C12P 2106, B29D 1100, C09K 300, C07K 100

Patent

active

059362561

ABSTRACT:
A collagen containing substrate comprising cattle basal membrane is conditioned by incubating the membranes in a mixture of pepsin, hyaluronidase and acetic acid and separating the collagen from the mixture. The mixture can include pepsin, hyaluronidase and a 0.5 M solution of acetic acid in 1:1:10 proportions. The incubation is conducted for about 10 hours at about 45.degree. C. After incubation, collagen is separated from the mixture by centrifuging at a speed of 5000 to 30000 rpms for about 30 minutes. The collagen is mixed with polymerizable monomer and polymerized to form a gel that can be ground to form an intraocular or contact lens.

REFERENCES:
patent: 5116615 (1992-05-01), Gokcen et al.
patent: 5162303 (1992-11-01), Goodman
patent: 5856120 (1999-01-01), Federov et al.

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