Method of preparing a biological material for use in ophthalmolo

Details Method of preparing a biological material for use in ophthalmolo Method of preparing a biological material for use in ophthalmolo

1997-01-29

1999-01-05

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

264 11, 264 138, 264 26, 264 28, 264 41, 351160H, 514 54, 530356, 527201, 523105, 523106, 523108, C12P 2106, B29D 1100

Patent

active

058561200

ABSTRACT:
A collagen containing substrate comprising cattle basal membrane is conditioned by incubating the membranes in a mixture of pepsin, hyaluronidase and acetic acid and separating the collagen from the mixture. The mixture can include pepsin, hyaluronidase and a 0.5M solution of acetic acid in 1:1:10 proportions. The incubation is conducted for about 10 hours at about 45.degree. C. After incubation, collagen is separated from the mixture by centrifuging at a speed of 5000 to 30000 rpms for about 30 minutes. The collagen is mixed with polymerizable monomer and polymerized to form a gel that can be ground to form an intraocular or contact lens.

REFERENCES:
patent: Re33997 (1992-07-01), Kuzma et al.
patent: 4024457 (1977-05-01), Kuettner et al.
patent: 4064008 (1977-12-01), Petersen et al.
patent: 4220724 (1980-09-01), Bert et al.
patent: 4268131 (1981-05-01), Miyata et al.
patent: 4876332 (1989-10-01), Tsilibary et al.
patent: 4894441 (1990-01-01), Menicagli
patent: 4983181 (1991-01-01), Civerchia
patent: 5103840 (1992-04-01), Kavoussi
patent: 5104957 (1992-04-01), Kelman et al.
patent: 5210182 (1993-05-01), Nasralish et al.
patent: 5246698 (1993-09-01), Leshchiner et al.
patent: 5424408 (1995-06-01), Reeders et al.
patent: 5476515 (1995-12-01), Kelman et al.

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