Method of preparing a biological material for use in ophthalmolo

Details Method of preparing a biological material for use in ophthalmolo Method of preparing a biological material for use in ophthalmolo

1998-11-17

2000-03-14

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

264 11, 264 138, 264 26, 264 28, 264 41, 351160H, 514 54, 530356, 527201, 523105, 523106, 523108, C12P 2106, B29D 1100

Patent

active

060371447

ABSTRACT:
A collagen containing substrate comprising cattle basal membrane is conditioned by incubating the membranes in a mixture of pepsin, hyaluronidase and acetic acid and separating the collagen from the mixture. The mixture can include pepsin, hyaluronidase and a 0.5M solution of acetic acid in 1:1:10 proportions. The incubation is conducted for about 10 hours at about 45.degree. C. After incubation, collagen is separated from the mixture by centrifuging at a speed of 5000 to 30000 rpms for about 30 minutes. The collagen is mixed with polymerizable monomer and polymerized to form a gel that can be ground to form an intraocular or contact lens.

REFERENCES:
patent: 5116615 (1992-05-01), Gokcen et al.
patent: 5162303 (1992-11-01), Goodman
patent: 5856120 (1999-01-01), Federov et al.

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