Method of preparing a B-1,4 glycan matrix containing a bound fus

Details Method of preparing a B-1,4 glycan matrix containing a bound fus Method of preparing a B-1,4 glycan matrix containing a bound fus

1992-04-08

1994-08-23

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 691, 435 6952, 435 697, 435 711, 4351723, 435177, 435195, 435200, 435209, 435803, 436530, 530808, 530814, 935 11, 935 14, C12N 1112, C12N 1500, C12P 2100

Patent

active

053407318

ABSTRACT:
A fusion protein that can function as a removable label is prepared containing a polypetide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with a .beta.-1,4 glycan matrix such as cellulose, the substrate binding region binds to the matrix to immobilize the polypeptide. The polypetide or fusion protein can be removed from the matrix with a protease capable of cleaving a specific protease cleavage site, or with a solution having a low ionic strength or a high pH. The fusion protein can be prepared by recombinant DNA technology.

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