Method of preparing 2-deoxyribose 5-phosphate

Details Method of preparing 2-deoxyribose 5-phosphate Method of preparing 2-deoxyribose 5-phosphate

2011-04-19

2011-04-19

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S252100, C435S131000

Reexamination Certificate

active

07927843

ABSTRACT:
The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.

REFERENCES:
Gijsen et al. , Chem. Rev. 1996, 96, 443-473.
Wong et al., J. Am. Chem. Soc., vol. 117, pp. 3333-3339 (1995).
Chen et al., J. Am. Chem. Soc., vol. 114, pp. 741-748 (1992).
Barbas et al., J. Am. Chem. Soc., vol. 112, pp. 2013-2014 (1990).
N. Itoh et al., Appl. Microbiol. Biotechnol, vol. 51, pp. 193-200, 1999.

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