Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1997-05-09
1999-09-28
McElwain, Elizabeth F.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
800278, 800279, 800280, 800286, 800295, 800298, 536 241, 536 245, 536 2372, 4353201, 435410, 435419, 435468, C12N 1505, C12N 1533, C12N 1564, A01H 100
Patent
active
059591811
ABSTRACT:
The present invention relates to a method of preparation of transgenic plants resistant to viral infections by introducing into the genoma of a host plant an antisense gene construct constituted by: the domain F of the subgenomic promoter of a viral RNA; a leader sequence of a viral ORF, downstream from said subgenomic promoter; the gene encoding a viral coat protein, downstream from said leader sequence; and the 3'-terminal region of a viral RNA, downstream from said gene. The present invention also relates to a recombinant vector comprising a promoter functional in a host plant, and, operably linked to this promoter, the antisense gene construct of the present invention.
REFERENCES:
patent: 5466788 (1995-11-01), Ahlquist et al.
Chemical Abstracts, vol. 113, No. 15, AN 127723, 1990, CN 1,033,645, Jul. 5, 1989.
Clayton C. Huntley, et al., vol. 192, pp. 290-297, "Minus Sense Transcripts of Brome Mosaic Virus RNA-3 Intercistronic Region Interfere with Viral Replication", 1993.
P. A. Powell, et al., Proceedings of the National Academy of Sciences of the USA, vol. 86, No. 18, pp. 6949-6952, "Protection Against Tobacco Mosaic Virus in Transgenic Plants that Express Tobacco Mosaic Virus Antisense RNA", Sep. 1989.
Maria Cuozzo, et al., Biotechnology, vol. 6, pp. 549-557, "Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or its Antisense RNA", May 1988.
M. Ali Rezaian, et al., Plant Molecular Biology, vol. 11, pp. 463-471, "Anti-Sense RNA's of Cucumber Mosaic Virus in Transgenic Plants Assessed for Control of the Virus", Apr. 19, 1988.
Arlen Nelson, et al., Gene, vol. 127, pp. 227-232, "Tobacco Mosaic Virus Infection of Transgenic Nicotiana Tabacum Plants is Inhibited by Antisense Constructs Directed at the 5' Region of Viral RNA", 1993.
Bruno Zaccomer, et al., Gene, vol. 136, pp. 87-94, "Transgenic Plants that Express Genes Including the 3' Untranslated Region of the Turnip Yellow Mosaic Virus (TYMV) Genome are Partially Protected Against TYMV Infection", 1993.
Z.R. Sung, et al., Science, "EMF, An Arabidopsis Gene Required for Vegetative Shoot Development", vol. 258, pp. 1645-1647, XP 002047279.
Joseph G. Atabekov, et al., vol. 309, pp. 23-24, "New Strategies for Construction of Virus Resistant Transgenic Plants", Apr. 1995.
Cuozzo et al. Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNa. BioTechnology. 6(5):549-557, May 1988.
Graves et al. Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus. Journal of Virology. 69(8):4746-4751, Aug. 1995.
Hayakawa et al. Nucleotide sequence analysis of cDNA encoding the coat protein of cucumber mosaic virus: genome organization and molecular features of the protein. Gene. 71:107-114, 1988.
Quemada et al. Nucleotide sequences of the coat protein genes and flanking regions of cucumber mosaic virus strains C and WL RNA 3. Journal of General Virology. 70:1065-1073, 1989.
Anderson et al. RNA 4 sequences from cucumber mosaic virus subgroups I and II. Gene. 161:293-294, 1995.
Smith et al. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature. 334:724-726, Aug. 1988.
Cellini Francesco
Grieco Pasquale Domenico
McElwain Elizabeth F.
Mehta Ashwin D.
Metapontum Agrobios S.c.r.l.
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