Method of preparation of purified alkaline protease

Details Method of preparation of purified alkaline protease Method of preparation of purified alkaline protease

1993-01-21

1995-08-08

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435221, 435816, C12N 956, C12N 954

Patent

active

054398177

ABSTRACT:
A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.

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