Method of preferential labelling of a phycobiliprotein with an a

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Metal containing – e.g. – chromoproteins – ferritin,...

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530370, 530395, 530345, 530807, 530403, 530404, 530405, 530408, 436501, 436536, 436546, 435961, 435964, A61K 3578, A61K 3580, C07K 1700, G01N 33531

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057836736

ABSTRACT:
A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.

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