Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...
Patent
1992-09-03
1993-12-21
Kim, Kay
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Plant proteins, e.g., derived from legumes, algae or...
530395, 530400, 530401, 530402, 530403, 530404, 530405, 530406, 530408, 530409, 530410, 530345, 530807, 435961, 435964, 436501, 436536, 436543, 436544, 436545, 436546, C07K 1510, C07K 1522, C07K 1702, G01N 33533
Patent
active
052722570
ABSTRACT:
A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.
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Cass Myron C.
Coulter Corporation
Kim Kay
LandOfFree
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