Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-04-15
1996-11-19
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 2, 435 721, 435 724, 435 725, 4352401, 436523, 436525, 436526, 436824, 210222, 210515, 210695, 422 44, G01N 33574
Patent
active
055761852
ABSTRACT:
A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including the non-selected population is separated from the particles with the selected population bound thereto. The particles can be heated for sterilization and endotoxin removal.
REFERENCES:
patent: 4115535 (1978-09-01), Giaever
patent: 4487700 (1984-12-01), Kanter
patent: 4910148 (1990-03-01), Sorensen et al.
patent: 5229268 (1993-07-01), Pry et al.
patent: 5238812 (1993-08-01), Coulter et al.
patent: 5256532 (1993-10-01), Melnicoff et al.
Patel et al., "Use of density perturbation to isolate immunologically distinct populations of cells," Journal of Immunological Methods, vol. 163, pp. 241-251 (1993).
Coulter Wallace H.
Russell Thomas R.
Schmittling Robert J.
Zwerner Robert K.
Coulter Corporation
Hutzell Paula K.
Kurz Warren W.
Winburn John T.
Wolski Susan C.
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