Method of plasmid recovery and apparatus for doing so

Details Method of plasmid recovery and apparatus for doing so Method of plasmid recovery and apparatus for doing so

2007-11-20

2007-11-20

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Vector, per se

C436S094000

Reexamination Certificate

active

10404580

ABSTRACT:
A process for the recovery of plasmids or other DNA from cells using a first filtration step to remove the cellular debris and other large cellular components and then an ultrafiltration step to capture the plasmids or other DNA on the surface of the ultrafiltration membrane where they may be recovered. An apparatus is also taught for enacting the process and comprises an upper microfiltration or coarse filtration membrane and a lower ultrafiltration membrane. The driving force may be the same for both filters or different and may be done sequentially or simultaneously.

REFERENCES:
patent: 5561064 (1996-10-01), Marquet et al.
patent: 6277648 (2001-08-01), Colpan
patent: WO9922020 (1999-06-01), None
patent: WO 00/66723 (2000-09-01), None
Lyddiatt et al. Biochemical recobery and purification of gene therapy vectors, Current Opinion in Biotechnology 1998 No. 9 pp177-185.

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