Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-02-12
1999-11-09
Marschel, Ardin H.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
424124, 435384, 435404, 536 2652, 536 2643, 71 8, 530417, A61K 3500, C07H 2100, C07H 2300, A23J 100
Patent
active
059817356
ABSTRACT:
A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.
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Hanak Julian A.J.
Hitchcock Anthony
Thatcher David R.
Varley Diane L.
Cobra Therapeutics Limited
Marschel Ardin H.
Tung Joyce
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