Method of permanent removal from cell suspension in a porous con

Details Method of permanent removal from cell suspension in a porous con Method of permanent removal from cell suspension in a porous con

1998-08-19

2000-09-26

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435374, 435 13, A01N 102

Patent

active

061240885

ABSTRACT:
A process and apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container. Convective-dispersion is applied via the combined action of gravity and pulsatile transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material such as that disclosed in U.S. Pat. No. 5,026,342 and U.S. Pat. No. 5,261,870, as incorporated herein by reference above. By using mechanical forces to aid the process of cryoprotectant removal, the benefits of slow cryoprotectant removal are retained while the actual removal time is markedly decreased.

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