Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1991-10-25
1995-06-13
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
435 4, 435 6, 436531, 436800, 436805, 436807, 436809, 522 1, 528491, 528494, 528495, 528497, 528498, 528502R, G01N 33543, G01N 33545
Patent
active
054242193
ABSTRACT:
Methods of performing an assay for biomolecules and solid supports for use in such assays are disclosed. An activated polymeric material is used as a solid support for binding biomolecules. In a preferred form, the support is made of a generally hydrophobic, nonporous polymer which has been activated by treatment with solvents or by mechanical means to enhance the binding characteristics of the support. The support can be made of a copolymer, such as a copolymer of polystyrene and polybutadiene. Detection of bound biomolecules is preferably performed by means of staining reactions and gray level scanning. Emulsifying agents, such as detergents, can be applied to the surface of the support to substantially inhibit additional materials from being bound to the support.
REFERENCES:
patent: 3639516 (1972-02-01), Sarfaty et al.
patent: 3663325 (1972-05-01), Uda et al.
patent: 3666733 (1972-05-01), Epton
patent: 3700609 (1972-10-01), Tregear
patent: 3847745 (1974-11-01), Axen et al.
patent: 3898087 (1975-08-01), Brutchen et al.
patent: 3966580 (1976-06-01), Janata et al.
patent: 4001583 (1977-01-01), Barrett
patent: 4119589 (1978-10-01), Horn et al.
patent: 4197361 (1980-04-01), Hoff et al.
patent: 4210418 (1980-07-01), Brown et al.
patent: 4217338 (1980-08-01), Quash
patent: 4235867 (1980-11-01), Thoma
patent: 4248965 (1981-02-01), Mochida et al.
patent: 4450231 (1984-05-01), Ozkan
patent: 4588680 (1986-05-01), Bucher et al.
patent: 4681782 (1987-07-01), Ozkan
patent: 4820644 (1989-04-01), Schafer et al.
patent: 4933410 (1990-06-01), Okrongly
patent: 4998284 (1991-03-01), Bacus et al.
patent: 5160600 (1992-11-01), Patel et al.
Webster, Owen W., "Science", vol. 251, 1991, pp. 887-893.
Bates, F. S., "Science", vol. 251, 1991, pp. 898-905.
Stanley et al, "Enzyme Amplification can Enhance Both Speed & Sensitivity of Immunoassays", J. Immunol. Meth., vol. 83, pp. 89-95, (1985).
Week 8716, Derwent Publications Ltd., London, GB; AN 87-111859 & JP,A, 62 056 862 (Kanebo KK) Mar. 12, 1987.
Patent Abstracts of Japan, vol. 7, No. 160 (P-210) (1305) Jul. 14, 1983 & JP,A, 58 70 164 (Toyo Boseki K.K.) Apr. 26, 1983.
Journal of Immunological Methods. vol. 83, Oct. 24, 1985, Amsterdam pp. 55-60, M. Sarkar et al. `Immobilization Of Antibodies On A New Solid Phase For Use In Elisa`.
J. D. Sedgwick, et al., "A solid phase immunoenzymatic technique for the enumeration of specific antibody secreting cells," J. Immunol. Meth., vol. 57, pp. 301-309 (1983).
S. F. De St. Groth, "The evaluation of limiting dilution assays," J. Immunol. Meth., vol. 49, pp. R11-R23 (1982).
E. Macy et al., "Enhanced ELISA: How to measure less than . . . 8 hours," F.A.S.E.B. Journal, vol. 2, pp. 3003-3009 (1988).
Chin Christopher L.
Cytech Biomedical, Inc.
Mates Edward I.
Scheiner Toni R.
Schwartz Robert M.
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