Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1985-01-17
1988-05-17
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
436540, 436544, 436546, 436800, 436809, 252 6251, 252 6252, 252 6254, G01N 33553, G01N 33533
Patent
active
047450770
ABSTRACT:
The invention concerns assays, such a immunoassays or two-site immunometric assays, performed using a labelled reagent and another reagent bound to magnetically attractable particles which are suspendable but insoluble in a liquid assay medium. After the labelled reagent has become partitioned between the liquid phase and the particles, in proportions which depend on the concentration of an analyte in a sample, the liquid phase is removed. Then the particles are re-suspended in another liquid medium, and the concentration of label observed. The method is particularly suitable for fluorescent and chemiluminescent' label systems, and can conveniently be performed in microtiter plates in which the wells are optically screened from one another, the observations being made from above or below the wells.
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patent: 3654090 (1972-04-01), Schuurs et al.
patent: 4177253 (1979-12-01), Davies et al.
patent: 4372745 (1983-02-01), Mandle et al.
patent: 4415700 (1983-11-01), Batz et al.
patent: 4438068 (1984-03-01), Forrest
patent: 4554088 (1985-11-01), Whitehead et al.
Charles Stephen A.
Edwards John C.
Holian John
Martin John K.
Amersham International plc.
Nucker Christine M.
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