Method of parentage testing in North American bison, Bison...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200

Reexamination Certificate

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07083925

ABSTRACT:
A method for determining parentage of North American bison or domestic cattle offspring. In the method an offspring is tested to determine the alleles present in that offspring for selected loci. A pool of potential parents is also tested to determine which alleles of these loci are present in each parent. The likelihood that any potential parent is the parent of the offspring may then be determined by looking for the presence or absence of an allele in the offspring that is present in the parent or by some comparable method. The method may also be used to exclude all potential parents in a pool. Sixteen loci which may be used in such an analysis are BM1225, BM1706, BM17132, BM1905, BM2113, BM4440, BM720, BMS1862, BMS410, BMS510, BMS527, RM372, BMS1172, BMS2639, BM3628 and BMS2325. The method used to select these loci is also disclosed.

REFERENCES:
Cattle Diversity Database , http://www.projects.roslin.ac.uk/cdiv/.
Parentage Testing Committee, American Association of Blood Banks (1998) Standards for Parentage Testing Laboratories, American Association of Blood Banks, Bethesda, MD.
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Validation of 15 microsatellites for parentage testing in North American bison, Bison bison and domestic cattle by R.D. Schnabel, T.J. Ward, and J.N. Derr. Animal Genetics 2000, 31, 360-366.

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