Surgery – Diagnostic testing – Detecting nuclear – electromagnetic – or ultrasonic radiation
Reexamination Certificate
2007-04-17
2010-10-05
Le, Long V (Department: 3768)
Surgery
Diagnostic testing
Detecting nuclear, electromagnetic, or ultrasonic radiation
C600S473000
Reexamination Certificate
active
07809431
ABSTRACT:
The present invention relates to a method of optically imaging at least one biological tissue, in particular to define areas of interest of tissue(s) to be analyzed by tomography.The method according to the invention comprises the following steps:a) introducing at least one fluorescent marker into the tissue(s);b) exciting the marker by incident light and detecting emission bands relating to fluorescence emitted by the marker in response to that excitation; thenc) analyzing the fluorescence in these emission bands; andthe step b) comprising:sequentially exciting said marker at n different incident excitation wavelengths λi, said marker being adapted to be excited by at least two of the wavelengths λiand to emit in response to each wavelength λia series Siof m simultaneous emission bands Bjhaving different maximum wavelengths λ′jthat are substantially the same from one series Sito another; anddetecting these series Siin order to deduce therefrom an estimate of the three-dimensional location of said marker in the tissue(s) and/or the mean absorption coefficients of the tissue(s) for the excitation wavelengths λi.
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Blanc Rolande
Heinrich Emilie
Peltie Philippe
Texier-Nogues Isabelle
Commissariat a l''Energie Atomique
Fontenot Nigel
Le Long V
Oblon, Spivak McClelland, Maier & Neustadt, L.L.P.
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