Method of obtaining gene product through the generation of trans

Details Method of obtaining gene product through the generation of trans Method of obtaining gene product through the generation of trans

1983-12-15

1989-09-26

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

4351723, 4352401, 435320, 935 6, 935 36, 935 53, 935 63, 935111, 800 1, C12P 2102, C12N 1500, C12N 500, C12N 100

Patent

active

048700096

ABSTRACT:
Mammalian genes that encode hormones are cloned and linked to strong promoter DNA sequences. The linked sequences are inserted in plasmids for amplification in prokaryotic cells, and multiple copies of the linked sequences are excised therefrom. Linked sequences are subsequently microinjected into fertilized eggs and the fertilized eggs are implanted into pseudo-pregnent females of the same species. As a result, transgenic animals are born having the linked sequences incorporated into their genomes and expressing the gene-encoded hormone. Because multiple copies of the linked sequences are frequently inserted and because production of the hormone is not limited to certain organs, as is the case with most endogenous hormones, the transgenic animals produce substantial amounts of the hormone. Hormone can be harvested from the living animal (and from its hormone-producing progeny) by extracting fluid, such as blood serum or ascites fluid, on a regular basis.

REFERENCES:
patent: 4276282 (1981-06-01), Sugimoto et al.
patent: 4419446 (1983-12-01), Howley et al.
patent: 4579821 (1986-04-01), Palmiter et al.
Wagner et al, PNAS USA, vol. 78, pp. 6376-6380 (Oct. 1981).
Wagner et al, PNAS USA, vol. 78, pp. 5061-5020 (Aug. 1981).
Gordon et al, PNAS USA, vol. 77, pp. 7380-7384, Dec. 1980.
Talkington et al, Promoters Structure and Function, edited by Rodriquez et al, Praeger Publishers, 307-331 (May 1981).
Palmiter et al, Nature, vol. 300, pp. 611-615, Dec. 16, 1982.
Brinster et al, Nature, vol. 296, pp. 39-42, Mar. 4, 1982.
Barta et al, PNAS USA, vol. 78, pp. 4867-4871, Aug. 1981.
Doehmer et al, PNAS USA, vol. 79, pp. 2268-2272, Apr. 1982.
Pavlakis et al, PNAS USA, vol. 78, pp. 7398-7402, Dec. 1981.
"Building Bigger Mice Through Gene Transfer", Science, vol. 218, Dec. 24, 1982, p. 1298.

Affiliated with

Also associated with

Rating

Method of obtaining gene product through the generation of trans does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of obtaining gene product through the generation of trans, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of obtaining gene product through the generation of trans will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-187300