Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides
Patent
1992-11-25
1995-08-01
Warden, Jill
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Synthesis of peptides
530334, 530350, A61K 3800, C07K 500, C07K 700, C07K 1700
Patent
active
054381193
ABSTRACT:
A method to obtain selected individual peptides or families thereof which have a target property and optionally to determine the amino acid sequence of a selected peptide or peptides to permit synthesis in practical quantities is disclosed. In general outline, the method of the invention comprises synthesizing a mixture of randomly or deliberately generated peptides using standard synthesis techniques, but adjusting the individual concentrations of the components of a mixture of sequentially added amino acids according to the coupling constants for each amino acid/amino acid coupling. The subgroup of peptides having the target property can then be selected, and either each peptide isolated and sequenced, or analysis performed on the mixture to permit its composition to be reproduced. Also included in the invention is an efficient method to determine the relevant coupling constants.
REFERENCES:
patent: 4510083 (1985-04-01), Blacklock et al.
patent: 4572894 (1986-02-01), Imahori et al.
patent: 4833092 (1989-05-01), Geysen
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5010175 (1991-04-01), Rutter et al.
patent: 5053454 (1991-10-01), Judd
patent: 5182366 (1933-01-01), Huebner et al.
patent: 5194392 (1993-03-01), Geysen
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5266684 (1993-11-01), Rutter et al.
Furka, A., Sebestyen, F., Asgedom, M., Dibo, G., Cornucopia of Peptides by Synthesis, Abstract, 14th International Congress of Biochemistry, Prague.
Furka, A., Sebestyen, F., Asredom, M., Dibo, G., More Peptides by Less Labour, Abstract, 10th International Symposium on Medicinal Chemistry, Budapest.
Furka, A., Sebestyen, F., Oulyon, J., Computer Made Electrophoretic Peptide Maps, 2nd International Conference on Biochemical Separations, Keszthely.
Furka, A., Sebestyen, F., Asgedom, M., Dibo, G., General method forrapid synthesis of multicomponent peptide mixtures, Int. J. Peptide Res. 37:487-493.
Creighton, T. E. Proteins, Wett, Freeman & Co., 1984, pp. 39-45.
Furka et al. (1988) 14th Int'l Congress of Biochemistry, vol. 5. Abstract FR:013.
Getzoff et al., "Mechanisms of Antibody Binding to a Protein" Science (1987) 235:1191-1196.
Geysen, et al., "Chemistry of Antibody Binding to a Protein" Science (1987) 235:1184-1190.
Houghten et al., "Generation and Use of Synthetic Peptide Combinatorial Libraries for Basic Research and Drug Discovery" Nature (1991) 354:84-86.
Lu, Heieng et al., Chem. Abs. 106(1):2453f, 1986.
Merrifield, "Solid Phase Synthesis" Angewandte Chemie (1985) 24(10):799-804.
Ragnarsson et al., "Studies on the Coupling Step in Solid Phase Peptide Synthesis. Further Competition Experiments and Attempts to Assess Formation of Ion Pairs" J. Org. Chem.; (1974) 39(26):3837-3841.
Stewart et al., Solid Phase Peptide Synthesis (1984) Pierce Chemical Co., pp. 34-38.
Zuckermann et al., "Identification of Highest-Affinity Selection from Equimolar Peptide Mixtures Generated by Robotic Synthesis" (Protos/Chiron Laboratories PNAS, 1992).
Houghten. P.N.A.S., 82:pp. 5131-5135, 1985.
Kovacs, Peptides, Gross et al, (ed). 1980, pp. 485-589.
Houghten, R. A., "General Method for the Rapid solid-Phase Synthesis of Large Numbers of Peptides: Specificity of Anti-gen-Antibody Interaction at the Level of Individual Amino Acids", Proc. Natl. Acad. Sci. USA, (1985, 82:5131-5135
Geysen et al., "Use of Peptide Synthesis to Probe Viral Antigens for Epitopes to a Resolution of a Single Amino Acid", Proc. Natl. Acad. Sci. USA, (1984), 81:3998-4002.
Stewart, Solid Phase Peptide Synthesis (2nd edition), Gross et al, (ed), Pierce Chemical Company (1984).
Kovacs, J., "Racemization and Coupling Rates of N.alpha.-Protected Amino Acids and Peptide Active Esters: Predictive Potential", The Peptides: Analysis, Synthesis, Biology, Gross et al. (ed), (1980), PP. 485-589.
Kemp et al., "Rate Constants for Peptide.rho. Nitrophenyl Ester Coupling Reactions in Dimethlyformamide. A Model for Steric Interactions in the Peptide Bond Forming Transition State", J. Org. Chem., (1974), 39(26):3841-3847.
Houghten et al., "Simultaneous Multiple Peptide Synthesis: The Rapid Preparation of Large Numbers of Discrete Peptides for Biological, Immunological, and Methodological Studies", Biotechniques, (1986), 4(6):522-524.
Berman, et al., "Rapid Optimization of Enzyme Substrates Using Defines Substrate Mixtures", The Journal of Biological Chemistry, (1992), 267:1434-1437.
Flynn et al., "Peptide-binding Specificity of the Molecular Chaperone BiP", Nature, (1991), 353:726-730.
Petithory et al., "A Rapid Method for Determination of Endoproteinase Substrate Specificity: Specificity of the 3C Proteinase from Hepatitis A Virus", Proc. Natl. Acad. Sci. USA, (1991), 88:11510-11514.
Lam et al., "A New Type of Synthetic Peptide Library for Identifying Ligand-Binding Activity", Nature, (1991), 354:82-82.
Rutter William J.
Santi Daniel V.
Bozicevic Karl
Davenport A. M.
The Regents of the University of California
Warden Jill
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