Method of obtaining a natural hydroxytyrosol-rich...

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

Reexamination Certificate

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C210S652000, C424S725000

Reexamination Certificate

active

08066881

ABSTRACT:
A process of obtaining a natural, bioactive concentrate, rich on hydroxytyrosol, from olive tree residues and subproducts using clean technologies is presented. These technologies comprise supercritical fluid extraction, nanofiltration and reverse osmosis which are used individually or in an integrated mode. The natural extract comprises hydroxytyrosol at a minimum concentration of 15% (mass fraction) and a maximum concentration of 98% (mass fraction). The hydroxytyrosol-rich concentrate exhibits anti-oxidant, anti-microbial, anti-inflammatory and anti-carcinogenic activities, which are superior to the activities observed for isolated hydroxytyrosol in equivalent concentration. The hydroxytyrosol-rich concentrate can be prepared in the form of solid particles, as an aqueous solution, in an emulsion or as lipidic based nanoparticles. Industrial application includes the food, pharmaceutical and cosmetics industries.

REFERENCES:
patent: 2003/0108651 (2003-06-01), Crea
patent: 1623960 (2006-02-01), None
patent: 2007230 (1989-06-01), None
patent: 02/18310 (2002-03-01), None
Allouche et al., Phenolic compounds with antioxidant activity from olive mill wastewaters, Journal de la Societe Chimique de Tunisie (2004), 6 (1), 33-43.
Chan et al, Preparation and characterization of nanofiltration membranes fabricated from poly (amidesulfonamide), and their application in water-oil separation (Journal of applied polymer science, 87: 1803-1810, 2003.
Skaltsounis, L., et al., “Minos Project Process development for an integrated olive mill waste management recovering natural antioxidants and producing organic fertilizer,” Internet Citation [Online] Apr. 2004, Retrieved from the Internet: URL:http://www.pharm.uoa.gr/minos/manualeng.pdf> [retrieved on Nov. 16, 2005].

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