Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1989-03-24
1994-09-13
Housel, James C.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2673, 536 2612, 436175, 436177, C07H 2300
Patent
active
053469992
ABSTRACT:
An automated apparatus is provided which implements a new method of extracting and purifying nucleic acids from cells without the use of centrifugation. In the method, a lysate is created by treating the cells with proteinase K in the presence of a lysis buffer having a high concentration of a salt. The lysate is mixed with a phenol-based solvent system, thereby creating an emulsion. The emulsion is heated to promote phase separation. Similarly, the rate of phase separation is also enhanced by increasing the surface area of the emulsion. Once the phase separation is complete, the lower organic phase is removed and the upper aqueous phase is repeatedly extracted with the phenol-based solvent a preselected number of times, and is finally extracted using chloroform. The remaining aqueous phase is then dialyzed to further purify and concentrate the nucleic acid solution. Two preferred embodiments of apparatus are presented to accomplish this extraction.
REFERENCES:
patent: 2967093 (1961-01-01), Raymond
patent: 3389133 (1968-06-01), Gutcho
patent: 3617547 (1971-11-01), Halff et al.
patent: 3847749 (1974-11-01), Smith et al.
patent: 3926738 (1975-12-01), Nyiri et al.
patent: 3935092 (1976-01-01), Bizot et al.
patent: 4264741 (1981-04-01), Friedman et al.
patent: 4460575 (1984-07-01), d'Hinterland et al.
patent: 4598049 (1986-07-01), Zelinka et al.
patent: 4985352 (1991-01-01), Julius et al.
patent: 5122599 (1992-06-01), Barnett et al.
Favloro et al. "Transcription Maps . . . Gel Mapping", Methods in Enzymology, 65, 718-723.
Nevins "Definition and Mapping . . . Transcription", Methods in Enzymology, 65, 768-775.
Hewlett-Packard Co. v. Bausch & Lomb Inc., 15USPQ2d. 1525. decided Jul. 30, 1990.
Adamson, Physical Chemistry of Surfaces, 4th Ed. pp. 471-474 Wiley-Interscience, 1982.
Maniatas et al.: Molecular Cloning, A Laboratory Manual, 1982, pp. 280, 366, 458-459, Cold Spring Harbor.
Weber:Physicochemical Process for Water Quality Control, Wiley-Interscience, 1972, p. 92.
Fujimoto, New Introduction to Surface Active Agents (Sanyo Chemical Industries, Ltd., Kyoto, 1985).
Coombs, Dictionary of Biotechnology (Elsevier, New York).
Wait et al., Applied and Environmental Microbiology, 46: 379-385 (1983).
Grossman and Moldave, eds., pp. 112-160 in Methods in Enzymology, vol. XII (Academic Press, New York, 1968).
Kirby, pp. 1-5 in Progress in Nucleic Acid Research and Molecular Biology, vol. 3 (Academic Press, New York, 1964).
Marmur, J. Mol. Biol., 3: 208-218 (1961).
Marmur, Methods in Enzymology, vol. VI, pp. 726-738 (1963).
Kirby, Biochem. J. 66: 495-503 (1957).
Cathcart Guy R.
Grossman Paul D.
Mayrand P. Eric
Nordman Eric S.
Whiteley Norman M.
Applied Biosystems Inc.
Bhat N.
Grubman Ronald E.
Housel James C.
Smith Joseph H.
LandOfFree
Method of nucleic acid extraction does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of nucleic acid extraction, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of nucleic acid extraction will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1121387