Plant husbandry – Process
Patent
1978-10-13
1980-05-27
Bagwill, Robert E.
Plant husbandry
Process
A01G 100
Patent
active
042043662
ABSTRACT:
This invention is a non-agricultural method for producing cotyledons of those plant species whose cotyledons have commercial value for the manufacture of useful products or for direct use as foodstuffs such as Theobroma cacao L. (cacao). The method involves three (3) distinct steps, namely, (1) proliferation of cotyledonary embryos by asexual embryogenesis through in vitro culture using a defined media; (2) the growth of the embryo in vitro in a media that will prevent premature germination, and (3) harvest of the in-vitro-grown cotyledon.
REFERENCES:
patent: 2747334 (1956-05-01), Routien et al.
patent: 3710512 (1973-01-01), Tamaki et al.
patent: 3710805 (1973-01-01), Tamaki et al.
patent: 3718153 (1973-02-01), Kobari et al.
Fertilization and Embryogeny--Cheesman, Ann. Bot. 41, 1927, pp. 107-126.
Culture in Vitro--Archibald, Nature, Feb. 20, 1954, pp. 351-352.
Initiation & Growth--Hall et al., Ann. Bot., (39), 1975, pp. 555-570.
Hormonal Control--Searles et al, Revista Theobroma, 6(3): 77-81, Jul.--Sep. 1976, Bahia, Brasil.
Growth of Oncobasidium--Prior, Journ. Gen. Microb., 1977-1979, pp. 219-222.
Tissue Culture Studies--Esan, Cocoa Res. Inst. of Nigeria, Ibadan, Nigeria, 1977, pp. 116-125.
Somatic Embryogenesis--Horticultural Reviews, 1979, AVI Publ. Co., Westport, Conn., pp. 1-77.
Janick Jules
Pence Valerie C.
Bagwill Robert E.
Nesbitt John R.
Purdue Research Foundation
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