Method of non-adhesive coating of a synthetic polymer based surf

Details Method of non-adhesive coating of a synthetic polymer based surf Method of non-adhesive coating of a synthetic polymer based surf

1998-01-26

2000-12-05

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

427307, 436531, 530413, 530815, C12N 1108, G01N 33545, G07K 1708

Patent

active

061565504

ABSTRACT:
A synthetic polymer based surface is coated with polymer particles without the use of an adhesive. A top surface layer is swollen or semidissolved by contacting with a solvent such as acetone, polymer particles are contacted with the surface to partially embed the particles in the surface layer, and the surface layer is dried. The polymer particles are either dry or in a slurry when contacted with the surface, and contact with the polymer particles may be simultaneous or subsequent to contacting with the solvent. The particles may be particles for chromatography, and may be derivatized before or after contacting with the surface such as by coupling an enzyme, DNA, an antibody or an antigen.

REFERENCES:
patent: 3993451 (1976-11-01), Verbeck
patent: 4465751 (1984-08-01), Kawamura et al.
patent: 4801665 (1989-01-01), Getman et al.
patent: 5098569 (1992-03-01), Stedronsky
patent: 5246737 (1993-09-01), Muradov
Merkos et al., Nucleic Acids Research, vol. No. 7, 1679-1684 (1992).
Derwent Abstracts 74-83716v (1994).
WPIDS Abstract 71-35653T (1969).

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