Method of multiplying a vector in E. coli host

Details Method of multiplying a vector in E. coli host Method of multiplying a vector in E. coli host

1994-09-16

1996-08-06

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352528, 4242001, 935 22, 935 29, 935 42, 935 73, C12N 1568, C12N 1520, C12N 1503, C12N 120

Patent

active

055433073

ABSTRACT:
A method of retaining or multiplying vector DNA stably in an Escherichia coli host comprising the step of culturing the host having both recA and recJ mutations containing said vector.

REFERENCES:
Thoms et al. "Suppression of the UV-Sensitive Phenotype of E. coli recF Mutants by recA & (srf) recA(tif) Mutations Requires recJ.sup.+ " J. Bacteriol. 170(8) 3675-3681 1988.
Kusano et al. "Plasmid Mediated Lethality & Plasmid Multimer Formation in an E. coli recBC sbcBC Mutant" J Mol. Biol. 209 623-634 1989.
Kolodne et al. "Genetic Recombination of Bacterial Plasmid DNA: Effect of RecF Pathway Mutations of Plasmid Recombination in E. coli " J Bacteriol. 163(3) 1060-1066 1985.
Lloyd et al. "Overlapping Functions of recD, recJ & recN Provide Evidence of 3 Epistatic Groups of Genes in E. coli Recombination & Repair" Biochimie 73 313-320 1991.
The Japanese Journal of Genetics, vol. 68 No. 5 Oct., 1993 including abstract of presentation (No. 2A15) by Ukita, T. Shimizu, H. and Ikeda, H., presented Sep. 18, 1993.

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