Method of multidimensional NMR with simultaneous plural...

Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...

Reexamination Certificate

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C324S307000, C324S308000, C324S314000

Reexamination Certificate

active

07605001

ABSTRACT:
There is disclosed an NMR measurement method of observing plural magnetization transfer patterns in one experiment and observing their respective time-modulated, evolved chemical shifts. In an earlier portion of a pulse sequence, a transition is made from in-phase magnetizations to antiphase magnetizations using RF pulses. In an intermediate portion of the sequence, the antiphase magnetizations are interchanged using 90° RF pulses. In a later portion of the sequence, a transition is made from the antiphase magnetizations to the in-phase magnetizations using other RF pulses. Simultaneously with the interchange of the magnetizations, evolution of chemical shifts is performed by a constant-time evolution technique. In the earlier and later portions of the pulse sequence, evolutions of the chemical shifts can be performed simultaneously because the magnetizations of two nuclei are in lateral magnetization state.

REFERENCES:
Yuxi Pang et al., “High-resolution detection of five frequencies in a single 3D spectrum: HNHCACO—a bidirectional coherence transfer experiment”,Journal of Biomolecular NMR, 11 (1998), pp. 185-190.

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