Method of monitoring HIV assembly and maturation

Details Method of monitoring HIV assembly and maturation Method of monitoring HIV assembly and maturation

2007-11-20

2007-11-20

Parkin, Jeffrey S. (Department: 1648)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

C424S208100

Reexamination Certificate

active

09800240

ABSTRACT:
The present invention provides methods to characterize the structure, stability, and intersubunit interfaces between the matrix, capsid, and nucleocapsid domains of the Gag polyprotein during HIV capsid assembly and maturation. A method of screening for compounds that promote or inhibit viral assembly and maturation is disclosed. A novel mass spectrometry based approach to measure hydrogen/deuterium exchange profiles is also disclosed. Quantitative data resulted from these studies may lead to well defined capsid assembly assays that can be adapted for rapid antiviral drug screening.

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