Method of modulating leukemic cell and eosinphil activity...

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Monoclonal antibody or fragment thereof

Reexamination Certificate

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C424S143100, C424S144100

Reexamination Certificate

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07427401

ABSTRACT:
A method of isolating a monoclonal antibody capable of inhibiting any one of IL-3, GM-CSF and IL-5 binding to the common receptor βc, or a monoclonal antibody capable of inhibiting the cytokines binding to a receptor analogous to βc. The method includes the steps of immunizing an animal with a cytokine receptor or portion of a cytokine containing the critical binding site which portion includes the extracellular domain 4 or analogous domain in the analogous common receptor or part thereof. Antibodies producing cells from the animal are then isolated and fused with a myeloma cell line and then screened for a cell line that produces an antibody of the desired type. A monoclonal antibody, or fragments thereof capable of inhibiting the binding of the cytokines IL-3, GM-CSF and IL-5 to the βcreceptor, and a hybridoma cell line producing the antibody are also claimed.

REFERENCES:
patent: WO 97/28190 (1997-08-01), None
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Watanabe et al. “Monoclonal Antibody Against the Common β Subunit (βc) of the Human Interleukin-3 (IL-3), IL-5, and Granulocyte-Macrophage Colony-Stimulating Factor Receptors Shows Upregulation of βcby IL-1 and Tumor Necrosis Factor-α”,Blood, 80(9): p. 2215-2220, 1992.
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