Method of modifying the DNA of an organism, DNA sequence, method

Details Method of modifying the DNA of an organism, DNA sequence, method Method of modifying the DNA of an organism, DNA sequence, method

1996-07-24

2000-01-18

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435440, 435455, 435471, 4352523, C12N 121

Patent

active

060157054

ABSTRACT:
Method of modifying DNA by subjecting the DNA to a mutation-inducing treatment. The method includes the steps of bringing the DNA to be mutated and a gene encoding a mutation-inducing non-DNA polymerase protein together in cells, growing the cells in the presence of a stress factor, and selecting the mutant cells which have developed a desirable trait in the presence of the stress factor.

REFERENCES:
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J. Cairns, J. Overbaugh, and S. Miller, 355 Nature 142-145 (1988).
G.J.W. Euverink, G.I. Hessels, J.W. Vrijbloed, J.R. Coggins, and L. Dijkhuizen, "Purification and characterization of a dual function 3-hydroquinate dehydratase from Amycolatopsis methanolica," 138 J. Gen. Microbiology 2449-2457 (1992).
G.J.W. Euverink, D.J. Wolters, and L. Dijkhuizen, "Prephenate dehydratase of the actinomycete Amycolatopsis methanolica: purification and characterization of wild-type and deregulated mutant proteins," 308 Biochem. J. 313-320 (1995).
P. Moretti, G. Hintermann, and R. Hutter, "Isolation and characterization of an extrachromosomal element from Nocardia mediterranei," 14 Plasmid 126-133 (1985).
J. W. Vrijbloed, J. Madon, and L. Dijkhuizen, "A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration," 176 J. Bacteriol. 7087-7090 (1994).

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