Method of mobilizing pluripotential hematopoietic stem cells wit

Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Blood

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424 9371, 424577, 424 852, 530413, C12N 1585, A61K 3514, A01N 6300

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056373236

ABSTRACT:
This invention provides a method of isolating an increased number of hematopoietic stem cells from a subject comprising a) administering interleukin-7 to the subject in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; and, b) isolating a population of leukocytes enriched for hematopoietic stem cells from the peripheral blood. Also provided is a method of transplanting an increased number of hematopoietic stem cells from a donor to a recipient to enhance repopulation of the recipient's hematopoietic and immune cells comprising a) administering interleukin-7 to the donor in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; b) isolating a population of leukocytes enriched for hematopoietic stem cells from the donor's peripheral blood; and, c) transplanting the isolated population of leukocytes enriched for hematopoietic stem cells to the recipient, thereby enhancing the repopulation of the recipient's hematopoietic and immune cells. Finally, the invention provides a method of improving engraftment of a bone marrow transplant or a peripheral blood leukocyte transplant from a donor to a recipient comprising administering interleukin-7 to the recipient following transplantation in an amount that enhances engraftment.

REFERENCES:
Faltyneck, CR et al. (1992). J. of Immunology. vol. 149 pp. 1276-1282.
Murphy WJ. et al. (1993). J. of Clinical Investigation. vol. 92 pp. 1918-1924.
Grzegorzewski et al., Administration of Recombinant Human Interleukin-7 to Mice Induces the Exportation of Myeloid Progenitor Cells From the Bone Marrow to Peripheral Sites, Blood, vol. 83, No. 2, pp. 377-385 (Jan. 15, 1994).
Grzegorzewski et al., rhIL7 Induces Extramedullary Hematopoiesis in Mice through the Exportation of Myeloid Progenitor Cells from the Bone Marrow to Peripheral Sites, J. Cell. Biochem. Suppl., Keystone Symposium 17B:63 (Jan. 31, 1993).
Damia et al., Administration of Recombinant Human IL-7 Alters the Frequency and Number of Myeloid Progenitor Cells in the Bone Marrow and Spleen of Mice, Blood, vol. 79, No. 5, pp. 1121-1129 (Mar. 1, 1992).
Morrissey et al., Administration of IL-7 to Mice with Cyclophosphamide-induced Lymphopenia Accelerates Lymphocyte Repopulation, J. Immunol., vol. 146, No. 5, pp. 1547-1552 (Mar. 1, 1991).
Jacobsen et al., Novel Role of Interleukin-7 in Myelopoiesis: Stimulation of Primitive Murine Hematopoietic Progenitor Cells, J. Exp. Med., vol. 178, No. 5, pp. 1777-1782 (Nov. 1, 1993).
Jackson et al., Mobilization of Hematopoietic Progenitors Following IL-7 Administration Post-chemotherapy, Blood, vol. 82, 10 Suppl. 1, 386A, (Dec. 3, 1993).
Armitage et al., Regulation of Human T Cell Proliferation by IL-7, J. Immunol., vol. 144, No. 3, pp. 938-941, (Feb. 1990).
Alderson et al., Interleukin 7 Enhances Cytolytic T Lymphocyte Generation and Induces Lymphokine-activated Killer Cells from Human Peripheral Blood, J. Exp. Med., vol. 172, pp. 577-587, (Aug. 1990).
Komschlies et al., In Vivo Tumoricidal and Immunohematological Effects of rhIL7 in Mice, Faseb J., vol. 5, No. 6, p. A 1772, Abstract No. 8137 (no date shown).
Komschlies et al., The In Vivo Effects of rhIL7 on T-Cell Number and Function in Normal or Tumor-Bearing Mice, J. Cell Biochem. Suppl., Keystone Symposia on Molecular and Cellular Biology: Cellular Immunity and the Immunotherapy of Cancer, Taos, NM, 17D:133, 1993.
Komschlies et al., Administration of Recombinant Human IL-7 to Mice Alters the Composition of B-Lineage Cells and T Cell Subsets, Enhances T Cell Function, and Induces Regression of Established Metastases, J. Immunol., 152:5776, (1994).
Bensinger et al., Autologous Transplantation With Peripheral Blood Mononuclear Cells Collected After Administration of Recombinant Granulocyte Stimulating Factor, Blood, vol. 81, No. 11, pp. 3158-3163, (Jun. 1, 1993).
Anne Kessinger, MD, Utilization of Peripheral Blood Stem Cells in Autotransplantation, Dept. of Inter. Med., University of Nebraska Med. Ctr., Omaha, vol. 7, No. 3, pp. 535-545 (Jun. 1993).
Meagher et al., Techniques of Harvesting and Cryopreservation of Stem Cells, James Graham Brown Cancer Center, University of Louisville (RCM, RHH), and others, vol. 7, No. 3, pp. 501-533 (Jun. 1993).

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