Method of microbial assay

Details Method of microbial assay Method of microbial assay

1983-06-06

1985-03-05

Turk, Arnold

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

250373, 2504612, 435 6, 435 40, 435808, 436 63, 436 94, 436164, 436177, C12Q 106, C12Q 108, G01N 2133

Patent

active

045031495

ABSTRACT:
A process for detecting the population count of microorganisms in an aqueous medium by means of ultraviolet spectrophotometry. The specimen is first filtered and the resulting filtrate then centrifuged to concentrate the microbial cells in a pellet layer. The microbial cells are then redispersed in an aqueous solution having a volume less than the volume of the original specimen and the absorption of ultraviolet light by the redispersed solution at wavelengths within the range of 260-280 nanometers is measured. The concentrated microbial cells may be lysed in order to release nucleic acid and protein components from the cellular wall structure.

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patent: 3745090 (1973-07-01), Chappelle et al.
patent: 3864212 (1975-02-01), Berkhan
patent: 3928139 (1975-12-01), Dorn
patent: 3971703 (1976-07-01), Picciolo et al.
patent: 4154821 (1979-05-01), Drouet et al.
patent: 4174772 (1979-11-01), Neuss et al.
Cherry, Plant Physiology, vol. 37, No. 5, pp. 670-678 (1962).

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