Method of measuring tumor suppressor gene p53

Details Method of measuring tumor suppressor gene p53 Method of measuring tumor suppressor gene p53

1997-04-03

2000-08-29

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 243, 536 2432, 536 2433, C12Q 168, C12N 1510, C07H 2104

Patent

active

061106712

ABSTRACT:
The instant invention provides a means for quantitatively determining the vel of tumor suppressor gene p53 by determination of level of messenger ribonucleic acid (mRNA) of the gene in a sample when compared with a prepared standard. The assay is quantitative in that the specific number of copies of the p53 mRNA in a sample may be derived from a curve from a standard of p53 RNA. The RNA used in preparation of a standard curve to quantitate RNA is generated using a plasmid which is part of the invention. In the assay, the RNA is produced by a protein (RNA polymerase) that reads the DNA message and manufactures an RNA copy. The RNA content of the transcribed sample is determined spectrophotometrically to measure the molar concentration. With prior knowledge of the molecular weight of the transcribed RNA and using Avogadro's number, the actual number of molecules of transcripted "control" p53 RNA can be determined

REFERENCES:
patent: 5726018 (1998-03-01), Pasternack
patent: 5811231 (1998-09-01), Farr et al.

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