Method of measuring the number of eumycete cells

Details Method of measuring the number of eumycete cells Method of measuring the number of eumycete cells

1984-04-12

1987-06-23

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 34, 435 39, 435911, C12Q 134

Patent

active

046752897

ABSTRACT:
A method of measuring the number of eumycete cells in a sample which comprises preparing a solution or suspension containing a sample of a medicine, food, drink, cosmetic or water, adding to said solution or suspension a 7-amino-4-methyl-coumarin derivative represented by formula (1): ##STR1## wherein R is an alkyl group, an allyl group, an aralkyl group, or a heterocyclic group, or R--CO-- is an amino acid or peptide residue, said derivative not inhibiting the hydrolysis of the amide bond of formula (1) by microorganism hydrolases contained in the samples; and measuring the fluorescence of 7-amino-4-methyl-courmarin released by the microorganism hydrolases.

REFERENCES:
patent: 3451893 (1969-06-01), Sanders
Godsey et al. Journal of Clinical Microbiology (1981), vol. 13, No. 3, pp. 483-490.
Derwent Publication 00141, Nov. 18, 1982.
Manual of Clinical Microbiology Chapter 55, 3rd Edition Lennett et al. (1980) page 566.

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