Method of measuring specific binding reaction with the aid of po

Details Method of measuring specific binding reaction with the aid of po Method of measuring specific binding reaction with the aid of po

1986-10-30

1988-02-16

Wan, Gene

Optics: measuring and testing

For size of particles

By particle light scattering

356364, G01N 1502, G01J 400

Patent

active

047251409

ABSTRACT:
A coherent light beam is made incident upon a cell via a polarizer. In the cell is contained a reaction liquid consisting of fine magnetic particles having an antibody coated thereon and a sample containing an antigen which is specifically reacted with the antibody on the particles. The particles are rotated in the reaction liquid by means of alternating magnetic fields having a frequency f.sub.0 and generated by coils arranged beside the cell. Light scattered by the particles is made incident upon a photodetector via a polarizer whose polarization plane is perpendicular to that of the analyzer. An output signal from the photodetector is synchronously detected by means of a reference signal having a frequency 2f.sub.0. Then a synchronously detected output signal represents an amount of the antigen contained in the sample.

REFERENCES:
patent: 4158234 (1979-06-01), Grandchamp
patent: 4521522 (1985-06-01), Lundstrom et al.
"Non-isotopic Immunoassay Methods", by Graham H. Beastall; Laboratory Practice, May 1985; pp. 74, 77, 78, and 81.
"Nephelometric Assay of Antigens and Antibodies with Latex Particles", by Grange Roch, and Quash; Journal of Immunological Methods, vol. 18; 1977; pp. 365-375.

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