Method of measuring oligonucleotide decomposing activity

Details Method of measuring oligonucleotide decomposing activity Method of measuring oligonucleotide decomposing activity

1996-03-07

1998-12-01

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

058436588

ABSTRACT:
The present invention provides an in situ monitoring of the decomposing activity against oligonucleotides target in a biological tissue. A single-chain oligonucleotide target comprises an appropriate number of nucleic acid bases to be examined with an energy donor and an energy acceptor respectively at its 5'- and 3'-terminals. Monitoring of the fluorescence changes of the target after injection into a biological tissue, particularly the fluorescent resonance energy transfer(FRET) phenomena between the energy donor and acceptor, indicates whether the oligonucleotide is not decomposed yet.

REFERENCES:
patent: 5037615 (1991-08-01), Kane
patent: 5607834 (1997-03-01), Bagwell
Ghosh et al, Nucleic Acid Res. 22(15):3155-3159, 1994.
Leonetti et al, PNAS 88:2702-2706 (Apr. 1991).
R. A. Cardullo, et al., "Detection of Nucleic Acid Hybridization by Nonradiative Fluorescence Resonance Energy Transfer", Proceedings of the National Academy of Sciences of the USA, Dec. 1, 1988, vol. 85, No. 23, pp. 8790-8794, XP000453537.
Uchiyama, et al., "Detection of Undergraded Oligonucleotides in Vivo by Fluorescence Resonance Energy Transfer", The Journal Of Biological Chemistry, vol. 27, No. 1, Issue of Jan. 5, pp. 380-384, 1996.
Ju et al, "Fluorescence Energy Transfer Dye-Labeled Primers for DNA Sequencing and Analysis", Pro. Natl Acad. Sci. USA, vol. 92, pp. 4347-4351, May 1995 Biophysics.

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