Method of measuring membrane fusion

Details Method of measuring membrane fusion Method of measuring membrane fusion

1984-05-24

1985-12-24

Richman, Barry S.

Chemistry: analytical and immunological testing

Optical result

With fluorescence or luminescence

424 2, 424 71, 436 63, 436829, G01N 2177, G01N 3352

Patent

active

045606650

ABSTRACT:
This method of measuring membrane fusions between liposomes made of phospholipid is characterized by the combination of ionophores, fluorescent dyes sensitive to the membrane potential (interior negative) and porins which are the pore-forming membrane proteins. By this method, the extent of liposome fusion can be determined easily and accurately.

REFERENCES:
patent: 4343782 (1982-08-01), Shapiro
Osmotic Swelling of Phospholipid Vesicles Causes them to Fuse with a Planar Phospholipid Bilayer Membrane, Cohen et al., Science, vol. 217, Jul. 30, 1982, pp. 458-460.
Interaction of Human Erythrocyte Ghosts of Liposomes with Polyethylene Glycol detected by Fluorescene Polarization, pp. 426-431.
Ohno et al., Biochem. and Biophys. Res. Comm., vol. 102 No. 1, Sep. 1981, Owen, Chemical Abstract 93:91333V, 1980.

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