Method of measuring melting temperature of nucleic acid

Details Method of measuring melting temperature of nucleic acid Method of measuring melting temperature of nucleic acid

1998-06-02

2000-02-29

Bennett, G. Bradley

Thermal measuring and testing

Transformation point determination

374 17, 436 94, 436 47, 436172, 435 6, G01N 2504, G01N 100, G12Q 168

Patent

active

060301155

ABSTRACT:
A method of measuring a melting temperature of a nucleic acid, which comprises a step of monitoring the fluorescent intensity of a mixture of a sample and a probe which is labeled with a fluorescent intercalative dye and contains a base sequence complementary to a specific nucleic acid in the sample, while varying the temperature of the mixture.

REFERENCES:
patent: 4544546 (1985-10-01), Wang et al.
patent: 5356776 (1994-10-01), Kambara et al.
patent: 5683657 (1997-11-01), Mian
patent: 5686244 (1997-11-01), Gudibande et al.

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