Method of measuring creatine kinase activity

Details Method of measuring creatine kinase activity Method of measuring creatine kinase activity

1981-09-16

1982-10-05

Castel, Benoit

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 26, C12Q 150

Patent

active

043528813

ABSTRACT:
A method and composition for use therein for measuring the activity of creatine kinase (hereinafter referred to as "CK") having good storage stability is disclosed. More particularly, a composition for measurement of CK activity which comprises as main components phosphoglycerate kinase (hereinafter referred to a "PGK") which catalyzes the following reaction 2 and glyceraldehyde-3-phosphate dehydrogenase (hereinafter referred to as "GAPDH") which catalyzes the following reaction 3. Reaction 1 is catalyzed by the creatine kinase.

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patent: 4162194 (1979-07-01), Pierre et al.
Cohn, M.; Biochimica et Biophysica Acta; vol. 20, pp. 92-99 (1956).
Koutras, J. A. et al.; Journal of Clinical Investigation; vol. 23, No. 2, pp. 323-331 (1964).
Dixon, M. et al., "Enzymes", Academic Press Inc. Pub., N.Y.; p. 569 (1964).

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