Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-07
1997-01-14
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435105, 435193, 435195, 4352571, 4352573, 435200, C12P 1912, C12N 112
Patent
active
055938698
ABSTRACT:
Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein.
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Nakano et al., "Purification And Some Properties Of A Trehalase From A Green Alga, Lobosphaera sp." Bioscience, Biotechnology, and Biochemistry, vol. 58, No. 8, pp. 1430-1434, Tokyo, Aug. 1994.
Nakano et al., "Formation Of Trehalose And Its 2-Deoxy Analogs Through Condensation By A Trehalase From Lobosphaera sp." Bioscience, Biotechnology, and Biochemistry, vol. 58, No. 8, pp. 1435-1438, Tokyo, Aug. 1994.
Kitahata Sumio
Moriwaki Masamitsu
Nakano Hirofumi
Washino Tsutomu
Lilling Herbert J.
Osaka
San-Ei Gen F.F.I
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