Method of making recombinant enzyme

Details Method of making recombinant enzyme Method of making recombinant enzyme

1998-12-22

2000-06-06

Achutamurthy, Ponnathapu

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4352525, 435832, 435839, 536 232, C12N 910, C12N 120, C12N 100, C07H 2104

Patent

active

060717335

ABSTRACT:
The present invention provides a polypeptide selected from a polypeptide of subunit (A) or a polypeptide of subunit (B) of a prenyl diphosphate synthetase; a DNA coding for the polypeptide; a recombinant vector comprising the DNA; a transformant transformed with the vector; and a method for preparing an active type prenyl diphosphate synthetase. Also disclosed is a method for preparing an active type enzyme on which a specific property has been conferred, comprising mixing polypeptides of the two subunits of a heterodimeric enzyme, the polypeptides being derived from different organisms and one of the polypeptides having the specific property.

REFERENCES:
Shimizu et al. (1996) Mechanism of isoprenoid chain elongation systems: Cloning and analysis medium-chain prenyl diphosphate synthases. Tennen Yuki Kagobutsu Toronkai Koen Yoshishu, 38th, 31-36 (abstract).
Ohnuma, et al., J. Bio. Chem., 269:20, pp. 14792-14797, May 20, 1994.
Math, et al., Proc. Natl. Acad. Sci. USA, 89, pp. 6761-6764, Aug. 1992.
Yoshida, et al., Biochem., 26:21, pp. 6840-6845,1987.

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