Method of making monoclonal antibodies using polymorphic transge

Details Method of making monoclonal antibodies using polymorphic transge Method of making monoclonal antibodies using polymorphic transge

1996-11-15

2000-04-25

Hauda, Karen

Multicellular living organisms and unmodified parts thereof and

Method of using a transgenic nonhuman animal to manufacture...

The protein is isolated or extracted from blood or serum

800 4, 424 931, 424 9321, 4241411, 435 7021, 435 702, 935 89, 514 2, A61K 4800, C12N 510, C12N 528, C12N 1509

Patent

active

060546328

ABSTRACT:
The invention relates to a method for making monoclonal antibodies having pre-defined specificity for an epitope characteristic of or unique to a single form of a polymorphic protein. The method includes constructing a first transgenic animal to express a first form of a polymorphic protein encoded by a first allele of a gene encoding the protein; constructing a second transgenic animal to express a second form of the polymorphic protein encoded by a second allele of the gene encoding the protein; and immunizing the first transgenic animal with cells from the second transgenic animal expressing the second form of the polymorphic protein to induce an immune response in the first transgenic animal yielding an antibody specific for an epitope peculiar to the second form of the polymorphic protein. The invention further includes hybridoma cells secreting a monoclonal antibody specific for the second form of the protein. The invention is particularly advantageous in the context of making monoclonal antibodies and derivative reagents specifically identifying polymorphic blood group proteins, such as the Duffy gp-Fy protein.

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