Method of making MHC-peptide complexes using metal chelate affin

Details Method of making MHC-peptide complexes using metal chelate affin Method of making MHC-peptide complexes using metal chelate affin

1994-04-14

1998-06-09

Caputa, Anthony C.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, 530415, C07K 122

Patent

active

057635854

ABSTRACT:
The present invention provides a method for the purification and characterization of MHC-peptide complexes useful in ameliorating immunological disorders, such as, for example, autoimmune diseases, allergic responses and transplant responses. The method disclosed is a one-step method based on the use of metal chelate affinity chromatography to separate the MHC-peptide complexes of interest from both uncomplexed MHC molecules and other endogenous MHC-peptide bound complexes.

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