Method of making lyophilized terminal deoxynucleotidyl transfera

Details Method of making lyophilized terminal deoxynucleotidyl transfera Method of making lyophilized terminal deoxynucleotidyl transfera

1982-07-26

1984-05-15

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435188, C12N 912, C12N 996

Patent

active

044488831

ABSTRACT:
Heat sensitive terminal deoxynucleotidyl transferase is stabilized by lyophilizing a solution of the enzyme, said solution prior to freeze-drying having a carefully controlled pH, an ionic concentration of at least 0.05 mole/liter and a protein concentration of greater than 0.3 gram/liter.

REFERENCES:
patent: 4339533 (1982-07-01), Chu
patent: 4342827 (1982-08-01), Atkinson et al.
Methods in Enzymology vol. XXII, pp. 32-38 (1971).

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