Liquid purification or separation – With means to add treating material – Chromatography
Patent
1989-04-10
1990-07-17
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
2105021, 210656, 502401, 502439, B01D 1508
Patent
active
049419749
ABSTRACT:
Dual zone reverse phase packing materials for use in liquid chromatographic blood serum analysis are prepared by contacting a porous support, such as porous silica, with a fluorocarbon silane, such as a perfluorobutylsilyl compound, to form a lipophobic phase in the external zone of the porous support, and, then contacting the porous support with a lipophilic silane, such as an octadecylsilyl compound, to form a lipophilic partitioning phase in the internal zone of the porous support. Ketal blocked diol groups, which later may be hydrolyzed to diol groups, are also attached to both zones of the packing materials. The dual zone reverse phase packing materials display a reduced degree of serum protein adsorption in the external zone while drug substances are retained and separated by the lipophilic partitioning phase in the internal zone.
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Dow Corning Corporation
Therkorn Ernest G.
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