Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-01
1998-03-17
Ketter, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 435364, C12N 1500
Patent
active
057285578
ABSTRACT:
A method for introducing a mutation into a desired site in Herpes Simplex Virus Type 1 uses a set of starting vectors, where each starting vector has a fragment of the substantially complete HSV-1 genome and also DNA which overlaps with a sequential genomic fragment contained in other starting vectors, so that upon co-transfection of a host cell, replication of viral DNA, and recombination, a mutated replicable virus is formed. The starting vector containing a gene which is to be mutated is replaced by a replacement vectors. The replacement vectors contain a copy of the mutated gene and overlapping DNA, and genomic DNA which was present in the starting vector. A host cell is transformed with the replacement vectors and the remaining starting vectors under conditions allowing replication of viral DNA and recombination to form a replicating mutated virus. In preferred embodiments, the protease gene is mutated.
REFERENCES:
patent: 5266489 (1993-11-01), Rey-Senelonge et al.
C. Cunningham et al., Virology 197, (1993) 116-124.
I. C. Deckman et al., Journal of Virology 66, (1992) 7362-7367.
C. L. Dilanni et al., Journal of Biological Chemistry 266, (1993) 25449-25454.
L. Matusick-Kumar et al., Journal of Virology 68, (1994) 5384-5394.
M. Gao et al., Journal of Virology 68, (1994) 3702-3712.
C. Addison et al., Virology 138 (1984) 246-259.
F. Liu et al., Proc. Natl. Acad. Sci. USA 89, (1992) 2076-2080.
P. L. Drake et al., The Journal of Biological Chemistry 269 (1994) 18708-18711.
C. L. Dilanni et al., The Journal of Biological Chemistry 268 (1993) 2048-2051.
V. G. Preston et al., Virology 186 (1992) 87-98.
L. Haarr et al., APMIS 102 (1994) 321-346.
W. Batterson et al., Journal of Virology 45 (1983) 397-407.
Desai et al., "The size and symmetry of B capsids of herpes simplex virus type 1 are determined by the gene products of the UL26 open reading frame", J. Virol., 68(9): 5365-5374, Sep. 1994.
Shih et al., "Expression of hepatitis B virus S gene by herpes simplex virus type 1 vectors carrying alpha-and beta-regulated gene chimeras", Proc. Nat. Acad. Sci. USA, 81: 5867-5870, Sep. 1984.
Sanders, et al., "Thymidine kinase muatnts of herpes simplex virus type 1", J. Gen. Virol., 63: 277-295, 1982.
Gaitanaris et al., "Reconstitution of an operon from overlapping fragments: use of lambda SV2 integrative cloning system", Gene 46 : 1-11, 1986.
McGeoch et al., "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1", J. Gen. Virol. 69: 1531-1574, 1988 .
Register R. Bruce
Shafer Jules A.
Giesser Joanne M.
Ketter James
Merck & Co. , Inc.
Tribble Jack L.
Yucel Irem
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