Method of making endoglucanase I

Details Method of making endoglucanase I Method of making endoglucanase I

1994-06-23

1996-06-25

Patterson, Jr., Charles

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

536 232, C12N 942, C12N 1556

Patent

active

055299199

ABSTRACT:
Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

REFERENCES:
patent: 4338397 (1982-07-01), Gilbert et al.
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4615974 (1986-10-01), Kingsman et al.
patent: 4794175 (1988-12-01), Nunberg et al.
Bailey, M. J. et al., Enzyme Microb. Technol. 3:153-157 (Apr. 1981).
Bhikhabhai, R. et al., "The cellulolytic enzymes of Trichoderma reesei as a system of homologous proteins," FEBS Letts. 167(2):301-308 (Feb. 1984).
Broome et al., Proc. Natl. Acad. Sci. USA 75 (6):2746-2749 (1978).
Chanzy, H. et al., FEBS Letts. 153 (1):113-118 (1983).
Chen, C. M. et al., Biotechnol. 5:74-77 (1987).
Churilova et al., "Purification and Proportions of Low-Molecular Weight Endoglucanase of the Cellulose Complex from Trichoderma koningii," Chemical Abstracts 93, Abs. No. 21382 (1980), (Abstract of Biokhimiya (Moscow), (1980), vol. 5 (4), pp. 669-678).
Enari, T.-M., Microbial Cellulases: 183-223, In: Microbiol, Enzymes and Biotechnologies, Wm. M. Fogart (ed.), Applied Science Publication, London and New York (1982).
Fagerstam, L. G. et al., FEBS Letts. 98:363-367 (1979).
Fagerstam, L. G. et al., FEBS Letts. 119:97-100 (1980).
Gong, Cheng-Shung, et al., Adv. Chem. Ser. 181:261-287 (1979).
Gritzali et al., "Purification and Characterization of endo 1, 4,-B-D glucanase and two exo-1,4 B-D glucanases from the Celluase System of Trichoderma reesei," Chemical Abstracts 93:18801.
Abst. No. 21423a, (Abstract of Diss. Abst. Int. B 1080, 40 (10):4783-4784) (1980).
Heijne, G. V., "A new method for predicting signal sequence cleavage sites," Nucl. Acids Res. 14(11): 4683-4689 (1986).
Henrissat, B. et al., Biotechnol. 3:722-726 (1985).
James, A. P. et al., Third Bioenergy R&D Seminar, Mar. 24-25, 1981, pp. 135-139.
Kubicek, C. P. et al., "The Trichoderma cellulase regulatory puzzle: From the Interior life of a secretory fungus," Enzyme Microb. Technol. 15:90-99 (Feb. 1993).
Mandels, M. et al., Appl. Microbiol. 21:152-154 (1971).
Mellor, J. et al., Gene 24:1-14 (1983).
Montenecourt, B. S., Trends in Biotechnology 1:156-161 (1983).
Nevalainen, K. M. H., App. Env. Microbiol. 41 (3):593-596 (1981).
Nummi, M. et al., Biochem. J. 215:677-683 (1983).
Penttila, M. E. et al., Gene 45:253-263 (1986).
Penttila, M. E. et al., Yeast 3:175-185 (1987).
Pettersson, G. et al., Institute of Biochemistry, University of Uppsala, III:39-42 (1981).
Picatagio et al., Abst. Annu. Meet of Am. Soc. Microbiol. 81, (1981), Abs. No. H19.
Raynal, A. et al., Mol. Gen. Genet. 195:108-115 (1984).
Saloheimo, M. et al., Gene 63:11-21 (1988).
Shoemaker, S. P. et al., Biochim. Biophys. Acta 523: 133-146 (1978).
Shoemaker, S. P. et al., Biochim. Biophys. Acta 523:147-161 (1978).
Shoemaker, S. et al., Bio/Technol. 1:691-696 (1983).
Shoemaker, S. et al., Biotechnol. 687-690 (1983).
Shoemaker et al., World Biotech. Rep. 2:593-600 (1984).
Sprey, B. et al., FEMS Microbiol. Letts. 18:217-222 (1983).
Teeri, T. et al., Bio/Technol. 1:696-699 (1983).
Thomsen, K. K., Carlsberg Res. Commun. 48:545-555 (1983).
van Arsdell, J. N. et al., Bio/Technol. 5:60-64 (1987).
van Tilbeurgh, H. et al., FEBS 1375 169 (2):215-218 (1984).

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