Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Reexamination Certificate
2007-05-15
2007-05-15
Devi, S. (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
C435S069300, C435S069100, C435S252200, C435S848000
Reexamination Certificate
active
10370522
ABSTRACT:
Disclosed herein are methods for making large amounts of highly pure colonization factors. The methods of the present invention differ from prior art methods in that host cells which express the colonization factor of interest are cultured in media comprising more than about 50 μg/l of an antibiotic, the media is centrifuged and then filtered with a 0.2 μm filter tangential flow cartridge and a 300,000 MW cut-off filter, and a divalent cation is added. As disclosed herein the colonization factors made by the method of the present invention may be used in pharmaceutical compositions and methods for treating or preventing enterotoxigenicEscherichia coliinfections.
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Supplementary Partial EP Search Report mailed from EPO May 6, 2005.
Cassels Frederick J.
Wood James F.
Arwine Elizabeth
Devi S.
United States of America as represented by the Secretary of the
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